In enzymology, an oxalate oxidase is an oxalate degrading enzyme that catalyzes the chemical reaction: The 3 substrates of this enzyme are oxalate, O2, and H+, whereas its two products are CO2 and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with oxygen as acceptor. The systematic name of this enzyme class is oxalate:oxygen oxidoreductase. Other names in common use include aero-oxalo dehydrogenase, and oxalic acid oxidase. This enzyme participates in glyoxylate and dicarboxylate metabolism. It uses Manganese as a cofactor.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes, , , and.