Middle Island Presbyterian Church (now known as Middle Island United Church of Christ) is a historic Presbyterian church at 271 Middle Country Road in Middle Island, Suffolk County, New York. The Federal style church building was constructed in 1837 by the Middle Island Presbyterian congregation with several later renovations and additions. The Presbyterian congregation built a new building in 1966. Later, a United Church of Christ congregation purchased the 1837 church building. The historic church building was added to the National Register of Historic Places in 2005.