This is a list of characters who serve as supporting cast of the Marvel Comics' Spider-Man.

Family

Tree

Note: This tree covers various different alternate realities to varying degrees.

Immediate family

Clones

Mary Jane's family

May Parker's Family

Spider-Man Family

Love interests

Peter Parker's love interests

Other continuity

Miles Morales' Love Interests

Other continuity

Ben Reilly's love interests

''Ben had Peter's memories of dating Betty Brant and Gwen Stacy, though not Mary Jane. This is due to the fact that Peter had not yet developed feelings for Mary Jane, at the time he was cloned.''

Peter Parker's supporting characters

Other

Daily Bugle

Horizon Labs

Parker Industries

Ben Reilly's supporting characters

Kaine's supporting characters

Gwen Stacy's family

Allies

Spider family

Other allies

Enemies

Alternative continuities

2099

Earth-928 a.k.a. Earth-616 circa 2099

Exiles

Omniverse (No Main Universe or Multiverse)

House of M

Earth-58163

Marvel Mangaverse

Earth-2301

Marvel Zombies

Earth-2149

MC2

Earth-982

Spider-Man: India

Earth-50101

Ultimate Marvel

Earth-1610

Spider-Man Unlimited

Earth-7831

Marvel's Spider-Man

Earth-1048

Marvel Cinematic Universe

Earth-199999

Supporting characters in other media

These supporting characters do not appear in the comics. They were created for various media series. Among them are: