The comic book character Spider-Man has had much media attention due to his popularity as a superhero, as have his villains. Here is a list of his primary villains that have undergone media attention such as in films, televisions, and video games as well as some villains who debuted in the TV series.

Film

This is a list of Spider-Man enemies that have been in Sam Raimi's Spider-Man film trilogy, Marc Webb's The Amazing Spider-Man films, the Marvel Cinematic Universe (MCU), and Sony's Spider-Man Universe. The movies in the original trilogy also introduces Dylan Baker as Dr. Curt Connors, the man who in the comics becomes the Lizard, but this transformation never took place during Raimi's tenure as director. Mendel Stromm was portrayed by actor Ron Perkins in Spider-Man, although he did not become a villain while Spider-Man 2 features Daniel Gillies as John Jameson, whose transformation into the Man-Wolf is not depicted in the film. Additionally, the Kingpin was featured prominently in the Mark Steven Johnson film Daredevil, where he was portrayed by Michael Clarke Duncan. In The Amazing Spider-Man, the Lizard is the villain. Irrfan Khan also portrays the antagonistic Dr. Ratha in that film. In The Amazing Spider-Man 2, Felicity Jones plays Felicia Hardy, the alter ego of Black Cat but her version of Black Cat was never shown. In Spider-Man: Homecoming, Michael Mando appears as Mac Gargan, but did not become Scorpion. In Spider-Man: Far From Home, Jake Gyllenhaal portrays Mysterio, who was originally to be played by Bruce Campbell in Raimi's canceled fourth film. Additionally, although the character has not appeared in an MCU film yet, Vincent D'Onofrio portrays the Kingpin in Marvel Television's Netflix series Daredevil and the Disney+ series Hawkeye, Echo, and Daredevil: Born Again. Live-action films Animated films

Television

A number of Spider-Man villains have made appearances in Spider-Man television series or other Marvel Comics related shows mostly in animation. Also, in Wolverine and the X-Men, there was a character that closely resembles Shadrac. Here is a listing in alphabetical order.

Sinister Six

A few of Spider-Man villains in other media have joined the group Sinister Six (or Insidious Six in Spider-Man: The Animated Series and Sinister Seven or Superior Sinister Six in Ultimate Spider-Man) to take down Spider-Man just like in the comics. Here is a list of villains who have joined. The numbers beside the supervillain with the parentheses in between them stand for their first meeting and second meeting and so on. There has never been a proper depiction of the original Sinister Six (Doctor Octopus, Mysterio, Vulture, Sandman, Kraven and Electro), but in most franchises, all of the members appear, mainly without joining.

Made-for-TV villains

These villains do not appear in the comics. They were created for various cartoon series. Among them are:

Spider-Man (1967)

Spider-Man (1981 TV series)

Spider-Man and His Amazing Friends (1981)

Spider-Man (1994 TV series)

Spider-Man: The New Animated Series (2003)

Ultimate Spider-Man (2012)

Video games

A number of Spider-Man villains have made appearances in Spider-Man video games series or other Marvel Comics related shows mostly in other games. Here is a listing in alphabetical order.

Novels, theatre and radio

Spider-Man villains that were introduced in novels, theatre productions and radio programs.

The Gentleman

For decades, the wealthy and misanthropic Gustav Fiers controlled a criminal organisation. Fiers had a lethal reputation in the criminal underworld, known for conducting elaborate plans to increase his own wealth, involved behind the scenes in various significant events in global history. Gustav Fiers eventually learned that Richard and Mary Parker, two agents under his employ, were double agents working for the United States government. When the Parkers were hired by Albert Malik, Fiers informed Malik of their disloyalty. Malik hired Gustav Fiers' brother, the Finisher, to kill the Parkers. The Finisher succeeded in doing so but, decades later, was killed in a battle with the Parkers' son, Spider-Man.

Lesser threats