This is a list of colleges and universities that are members of Division I, the highest level of competition sponsored by the National Collegiate Athletic Association (NCAA). Currently, there are 364 institutions classified as Division I (including those in the process of transitioning from other divisions), making it the second largest division by school count in the NCAA. An additional 206 institutions in one of the NCAA's other two divisions compete or will compete in Division I in at least one sport. All colleges and universities on this list are located in the United States; all states (except Alaska) plus the District of Columbia are represented by full members. Information in this list represents the current 2024–25 seasons.

Full members

These schools are full members of Division I, meaning they either have finished the process of joining Division I or were members of its predecessor, the University Division.

Transitioning members

These schools are at some point in the process of joining Division I, but as of 2023, have not yet finished the process and thus aren't yet full members. Unless stated otherwise, all transitions begin and end on July 1 of a given year.

Approved reclassifications

All moves between divisions require NCAA approval before commencing. These schools gained this approval and as such have begun their transitions.

Partial members

These schools are not members of Division I, but rather are members of Division II or Division III that compete in one or more sports at the Division I level. In many cases, these institutions play in Division I because their divisions do not have championships for a particular sport. Four institutions, all Division II members, fielded teams in a Division I sport for the first time in the 2024–25 school year. Rockhurst added men's volleyball; Menlo, Roosevelt, and Vanguard, which played that sport in the NAIA in 2023–24, aligned fully with the NCAA.

Current partial members

Future partial members

These schools that are members of other divisions, or of the NAIA, have announced their intentions to field one team at the Division I level sometime after the 2024–25 school year. NAIA members listed here have either started transitions to the NCAA or have announced plans to do so. Years of joining reflect calendar years. For schools that will play only spring sports at the D-I level, the calendar year of joining is the year before the first season of competition.

Former full members

This list includes institutions that sponsored athletic programs that competed at the highest level in the NCAA (Division I 1973-present, University Division 1957-1973). Schools that were deemed major schools in athletics before 1957 are not included in this list.