As of 2022, there are 588 National Wildlife Refuges in the United States, with the addition of the Green River National Wildlife Refuge. Refuges that have boundaries in multiple states are listed only in the state where the main visitor entrance is located. The newest refuge replaces the Cherry Valley National Wildlife Refuge in Pennsylvania. Refuges are listed regardless of whether or not they are open to the public, which many are not. The United States is divided into eight regions for administrative purposes:

Alabama

Alaska

American Samoa

Arizona

Arkansas

California

Colorado

Connecticut

Delaware

District of Columbia

Florida

Georgia

Guam

Hawaii

Idaho

Illinois

Indiana

Iowa

Kansas

Kentucky

Louisiana

Maine

Maryland

Massachusetts

Michigan

Minnesota

Mississippi

Missouri

Montana

Nebraska

Nevada

New Hampshire

New Jersey

New Mexico

New York

North Carolina

North Dakota

Northern Mariana Islands

Ohio

Oklahoma

Oregon

Pennsylvania

Puerto Rico

Rhode Island

South Carolina

South Dakota

Tennessee

Texas

U.S. Minor Outlying Islands

Utah

Vermont

Virgin Islands (U.S.)

Virginia

Washington

West Virginia

Wisconsin

Wyoming