The following is a list of characters from the Japanese manga series Gunslinger Girl. Set in modern-day Italy, the series revolves around the Social Welfare Agency, a government-funded organisation which is supposed to provide advanced medical care to those in need. This, however, is a cover for a far different agenda: the patients, adolescent girls who have survived traumatic events, are brainwashed into forgetting their past, turned into cyborgs, trained in the use of weapons and used as assassins to battle enemies of the State. Each girl is assigned to an adult whose job is to provide the girl with training and act as a mentor and authority figure. The relationship between the cyborg-girls and their "handlers" forms the basis of much of the plot and varies from the affectionate to the indifferent with varying degrees of results.

Child cyborg assassins

First Generation

Second Generation

Cyborg handlers

Social Welfare Agency staff

Section 1

Section 2

Technology department

Public Security

Others

Five Republics Faction members

The Five Republics Faction is a covert radical nationalist and right-wing group dedicated to the independence of northern Italy, as well as the opposition of globalization in Italy. This group is also referred to as Padania, after a valley in northern Italy formed by the Po River.