This list contains a list of EC numbers for the third group, EC 3, hydrolases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology. All official information is tabulated at the website of the committee. The database is developed and maintained by Andrew McDonald.

EC 3.1: Acting on Ester Bonds

EC 3.1.1: Carboxylic Ester Hydrolases

EC 3.1.2: Thioester Hydrolases

EC 3.1.3: Phosphoric Monoester Hydrolases

EC 3.1.4: Phosphoric Diester Hydrolases

EC 3.1.5: Triphosphoric Monoester Hydrolases

EC 3.1.6: Sulfuric Ester Hydrolases

EC 3.1.7: Diphosphoric Monoester Hydrolases

EC 3.1.8: Phosphoric Triester Hydrolases

EC 3.1.11: Exodeoxyribonucleases Producing 5'-Phosphomonoesters

EC 3.1.13: Exoribonucleases Producing 5'-Phosphomonoesters

EC 3.1.14: Exoribonucleases Producing 3'-Phosphomonoesters

EC 3.1.15: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters

EC 3.1.16: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters

EC 3.1.21: Endodeoxyribonucleases Producing 5'-Phosphomonoesters

EC 3.1.22: Endodeoxyribonucleases Producing 3'-Phosphomonoesters

EC 3.1.23: and EC 3.1.24 now EC 3.1.21.3, EC 3.1.21.4 and EC 3.1.21.5

Deleted sub-subclasses.

EC 3.1.25: Site-Specific Endodeoxyribonucleases Specific for Altered Bases

EC 3.1.26: Endoribonucleases Producing 5'-Phosphomonoesters

EC 3.1.27: Endoribonucleases Producing 3'-Phosphomonoesters

EC 3.1.30: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters

EC 3.1.31: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters

EC 3.2: Glycosylases

EC 3.2.1: Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds

EC 3.2.2: Hydrolysing N-Glycosyl Compounds

EC 3.2.3: Hydrolysing S-Glycosyl Compounds

Deleted sub-subclass

EC 3.3: Acting on Ether Bonds

EC 3.3.1: Thioether and trialkylsulfonium hydrolases

EC 3.3.2: Ether Hydrolases

EC 3.4: Acting on peptide bonds – Peptidase

EC 3.4.1 α-amino acyl peptide hydrolases (discontinued)

EC 3.4.2 Peptidyl amino acid hydrolases (discontinued)

EC 3.4.3: Dipeptide hydrolases (deleted sub-subclass)

EC 3.4.4 Peptidyl Peptide Hydrolases (discontinued)

EC 3.4.11 Aminopeptidases

EC 3.4.12 Peptidylamino-acid hydrolases or acylamino-acid hydrolases (deleted sub-subclass)

EC 3.4.13 Dipeptidases

EC 3.4.14 Dipeptidyl peptidases and tripeptidyl peptidases

EC 3.4.15 Peptidyl dipeptidases

EC 3.4.16 Serine type carboxypeptidases

EC 3.4.17 Metallocarboxypeptidases

EC 3.4.18 Cysteine type carboxypeptidases

EC 3.4.19 Omega peptidases

EC 3.4.21: Serine proteases

EC 3.4.22 Cysteine proteases

EC 3.4.23 Aspartic endopeptidases

EC 3.4.24: Metallopeptidases

EC 3.4.25 Threonine endopeptidases

EC 3.5: Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.1: In linear amides

3.5.2: In cyclic amides

3.5.3: In linear amidines

3.5.4: In cyclic amidines

3.5.5: In nitriles

3.5.99: In other compounds

EC 3.6: Acting on acid anhydrides

3.6.1: In phosphorus-containing anhydrides

3.6.2: In sulfonyl-containing anhydrides

3.6.3: Acting on acid anhydrides to catalyse transmembrane movement of substances

3.6.4: Acting on acid anhydrides to facilitate cellular and subcellular movement

3.6.5: Acting on GTP to facilitate cellular and subcellular movement

EC 3.7: Acting on carbon-carbon bonds

EC 3.7.1: In ketonic substances

EC 3.8: Acting on halide bonds

EC 3.8.1: Acting on halide bonds

3.8.2: In phosphorus-halide compounds (deleted sub-subclass)

EC 3.9: act on phosphorus-nitrogen bonds

EC 3.9.1: Acting on phosphorus-nitrogen bonds (only sub-subclass identified to date)

EC 3.10: Acting on sulfur-nitrogen bonds

EC 3.10.1: Acting on sulfur-nitrogen bonds (only sub-subclass identified to date)

EC 3.11: Acting on carbon-phosphorus bonds

EC 3.11.1: Acting on carbon-phosphorus bonds (only sub-subclass identified to date)

EC 3.12: Acting on sulfur-sulfur bonds

EC 3.12.1: Acting on sulfur-sulfur bonds (only sub-subclass identified to date)

EC 3.13: Acting on carbon-sulfur bonds

EC 3.13.1: Acting on carbon-sulfur bonds (only sub-subclass identified to date)