The Leader of His Majesty's Opposition in Victoria is the leader of the largest political party in parliament that is not in government. They are always a member of the Legislative Assembly. Prior to 1904, opposition to the government of the day was less organised. Thus, the Victorian Parliamentary Record does not designate Leaders of the Opposition before then. The leader acts as the public face of the opposition, leading the opposition on the floor of parliament. They act as a chief critic of the government and ultimately attempt to portray the opposition as a feasible alternate government. The office is currently held by John Pesutto after his election to the position of leader of the Liberal Party in December 2022.

List of leaders of the opposition in Victoria

This is an list of leaders of the opposition in Victoria.