Ein HaBesor is a moshav in southern Israel. Located in the Hevel Eshkol area of the north-western Negev desert near the border with the Gaza Strip and around a kilometre from Magen, it falls under the jurisdiction of Eshkol Regional Council. In it had a population of.

History

Ein Besor was an Egyptian First Dynasty staging post along the "ways of Horus" trade route in the northern Negev. The staging post was contemporary with Tell es-Sakan. The modern moshav was established in 1982. Some of the residents were from Sadot, an Israeli settlement in the Sinai Peninsula evacuated after signing of the Egypt–Israel peace treaty. On 7 October 2023, Ein HaBesor was attacked by Hamas fighters. The moshav's civil defense team, with only four M16 rifles, repelled the attack.