The Women's 400 metres at the 2000 Summer Olympics as part of the athletics programme was held at Stadium Australia on Friday 22 September, Saturday 23 September, Sunday 24 September, and Monday 25 September 2000. The winning margin was 0.47 seconds. The top three runners in each of the initial seven heats automatically qualified for the second round. The next eight fastest runners from across the heats also qualified for the second round. The top four runners in each of the four second round heats automatically qualified for the semi-final. The top four runners in each semi-final automatically qualified for the final. There were a total number of 59 participating athletes.

Records

These were the standing world and Olympic records (in seconds) prior to the 2000 Summer Olympics.

Medals

Results

All times shown are in seconds.

Qualifying heats

Round 1

Overall Results Round 1

Round 2

Overall Results Round 2

Semi-finals

Overall results: Semi-finals

Final