María José Martínez Sánchez defeated Jelena Janković in the final, 7–6(7–5), 7–5 to win the women's singles tennis title at the 2010 Italian Open. Martínez Sánchez was unseeded in the main draw. Dinara Safina was the defending champion, but lost in the second round to Alexandra Dulgheru. Top seed and world No. 1 Serena Williams made it to the semifinals, but was edged by Janković after failing to convert a match point. Unseeded and former world No. 1 Ana Ivanovic also made a surprise run into the semifinals by beating two top 10 players and a top 15 player, but failed to defeat Martínez Sánchez.

Seeds

The top eight seeds receive a bye into the second round. • # 🇺🇸 Serena Williams (semifinals) • # Caroline Wozniacki (third round) • # 🇷🇺 Dinara Safina (second round) • # 🇺🇸 Venus Williams (quarterfinals) • # 🇷🇺 Svetlana Kuznetsova (second round) • # 🇷🇺 Elena Dementieva (third round) • # 🇷🇸 Jelena Janković (final) • # 🇵🇱 Agnieszka Radwańska (third round) • # 🇧🇾 Victoria Azarenka (second round) • # 🇦🇺 Samantha Stosur (withdrew due to fatigue) • # 🇧🇪 Yanina Wickmayer (third round) • # 🇮🇹 Flavia Pennetta (second round) • # 🇮🇹 Francesca Schiavone (second round) • # 🇷🇺 Nadia Petrova (quarterfinals) • # 🇷🇺 Vera Zvonareva (first round) • # 🇮🇱 Shahar Pe'er (third round)

Draw

Finals

Top half

Section 1

Section 2

Bottom half

Section 3

Section 4