The 2010 Bonnaroo Music and Arts Festival was held on June 10–13, 2010. The line-up was announced Tuesday, February 9, although the original line-up release date was scheduled for February 2. It was broadcast live on YouTube. Pre-sale tickets went on sale November 27, 2009.

Line-up

The majority of artists were announced on Bonnaroo's official MySpace and Twitter pages over a span of nine hours. On March 4, Ween, John Butler Trio, Umphrey's McGee, Galactic, The Gossip, and Circa Survive were added to the line-up. On March 11, it was announced that Conan O'Brien would stop at the festival during The Legally Prohibited from Being Funny on Television Tour. Recordings of Tenacious D's performance on June 11 were released for free on the Live Music Archive.

Thursday, June 10

(artists listed from earliest to latest set times)

Friday, June 11

(artists listed from earliest to latest set times)

Saturday, June 12

(artists listed from earliest to latest set times)

Sunday, June 13

(artists listed from earliest to latest set times)