Yuki Bhambri was the defending champion, but did not compete in the Juniors this year. The boys' singles tournament of the 2010 Australian Open started in the week of 25 January, the second week of the main tournament. Tiago Fernandes won in the final 7–5, 6–3, against Sean Berman.

Seeds

• # 🇸🇪 Daniel Berta (third round) • # 🇫🇷 Gianni Mina (semifinals) • # 🇦🇺 Jason Kubler (third round) • # 🇺🇸 Mitchell Frank (third round) • # Huang Liang-chi (second round) • # 🇭🇺 Márton Fucsovics (semifinals) • # 🇩🇪 Kevin Krawietz (first round) • # 🇩🇪 Dominik Schulz (second round) • # 🇸🇰 Filip Horanský (third round) • # 🇧🇷 Guilherme Clézar (quarterfinals) • # 🇦🇷 Renzo Olivo (third round) • # 🇯🇵 Yasutaka Uchiyama (first round) • # 🇧🇪 Arthur de Greef (third round) • # 🇧🇷 Tiago Fernandes (champion) • # 🇫🇮 Henri Laaksonen (first round) • # 🇸🇰 Jozef Kovalík (second round) Note: Daniel Berta was, at the end of 2009, ranked world no. 1 among the juniors, but forgot to apply for a place in the tournament; he was given a wild card for the qualification round, through which he qualified for the main tournament.

Draw

Finals

Top half

Section 1

Section 2

Bottom half

Section 3

Section 4