The 2008–09 Czech First League, known as the Gambrinus liga for sponsorship reasons, was the sixteenth season of Czech Republic's top-tier of football. It began on 2 August 2008 and ended on 30 May 2009. Slavia Prague were the defending champions. Slavia secured their 17th title overall after a 3–1 win away against Viktoria Žižkov in 28th round. They were the first club to defend Czech title since the 2000–01 season.

Promotion and relegation

Most and Bohemians 1905 were relegated to the second division after finishing last and second to last, respectively, in the previous season. FK Bohemians Prague (Střížkov) (as champions) and 1. FK Příbram (as runners-up) were promoted from the second division.

Stadia and locations

Managerial changes

League table

Results

Top goalscorers

Final standing