Jelena Janković defeated Svetlana Kuznetsova in the final, 7–5, 6–1 to win the women's singles tennis title at the 2007 Italian Open. It was her second Tier I title. Martina Hingis was the reigning champion, but did not participate this year due to a hip injury.

Seeds

The top eight seeds received a bye into the second round. • # 🇫🇷 Amélie Mauresmo (second round) • # 🇷🇺 Svetlana Kuznetsova (finals) • # 🇷🇸 Jelena Janković (champion) • # 🇨🇿 Nicole Vaidišová (withdrew due to a right wrist injury) • # 🇷🇺 Nadia Petrova (third round; retired due to low back pain) • # 🇷🇺 Dinara Safina (quarterfinals) • # 🇷🇺 Anna Chakvetadze (third round) • # 🇺🇸 Serena Williams (quarterfinals) • # 🇸🇰 Daniela Hantuchová (semifinals) • # 🇷🇺 Elena Dementieva (quarterfinals) • # 🇮🇱 Shahar Pe'er (third round) • # 🇷🇸 Ana Ivanovic (withdrew due to an ankle injury) • # 🇨🇳 Li Na (withdrew due to gastrointestinal illness) • # Patty Schnyder (semifinals) • # Katarina Srebotnik (second round) • # 🇮🇹 Tathiana Garbin (second round)

Draw

Finals

Top half

Section 1

Section 2

Bottom half

Section 3

Section 4