The first round of the 2007–08 UEFA Cup began on 20 September 2007, which narrowed clubs down to 40 teams in preparation for the group stage.

Summary

<section begin=R1 /> The draw, which was conducted by UEFA General Secretary [David Taylor](https://bliptext.com/articles/david-taylor-scottish-football) and [Gérard Houllier](https://bliptext.com/articles/g-rard-houllier), the winning coach in the [2000–01 tournament](https://bliptext.com/articles/2000-01-uefa-cup), was held on Friday, 31 August 2007 at 13:00 CET in Monaco. The matches were played on 20 September and 4 October 2007. !colspan="5"|Group 1 !colspan="5"|Group 2 !colspan="5"|Group 3 !colspan="5"|Group 4 !colspan="5"|Group 5 !colspan="5"|Group 6 !colspan="5"|Group 7 !colspan="5"|Group 8 1 This match was played at [Panthessaliko Stadium](https://bliptext.com/articles/panthessaliko-stadium) in [Volos](https://bliptext.com/articles/volos) because [AEL](https://bliptext.com/articles/athlitiki-enosi-larissa-f-c)'s ground ([Alkazar Stadium](https://bliptext.com/articles/alkazar) in [Larissa](https://bliptext.com/articles/larissa)) would not meet UEFA standards.<section end=R1 />

First leg

Second leg

Panathinaikos won 5–1 on aggregate. ''1. FC Nürnberg 2–2 Rapid București on aggregate.

1. FC Nürnberg won on away goals.'' Tottenham Hotspur won 7–2 on aggregate. Lokomotiv Moscow won 5–1 on aggregate. Helsingborg won 8–6 on aggregate. Atlético Madrid won 9–0 on aggregate. Hamburger SV won 4–1 on aggregate. Zürich won 4–2 on aggregate. Austria Wien won 4–2 on aggregate. ''Dinamo București 2–2 Elfsborg on aggregate. Elfsborg won on away goals.'' ''Aberdeen 1–1 Dnipro Dnipropetrovsk on aggregate. Aberdeen won on away goals.'' ''Toulouse 1–1 CSKA Sofia on aggregate. Toulouse won on away goals.'' ''2–2 on aggregate. AaB won on away goals.'' Spartak Moscow won 8–1 on aggregate. ''Sparta Prague 0–0 Odense on aggregate. Sparta Prague won 4–3 on penalties.'' Basel won 8–1 on aggregate. ''Dinamo Zagreb 3–3 Ajax on aggregate. Dinamo Zagreb won on away goals.'' Villarreal won 6–1 on aggregate. Panionios won 2–1 on aggregate. Zenit Saint Petersburg won 4–1 on aggregate. Copenhagen won 3–2 on aggregate. AEK Athens won 3–1 on aggregate. Hapoel Tel Aviv won 1–0 on aggregate. ''Brann 2–2 Club Brugge on aggregate. Brann won on away goals.'' Bayern Munich won 3–0 on aggregate. Galatasaray won 7–4 on aggregate. Everton won 4–3 on aggregate. ''Getafe 3–3 Twente on aggregate. Getafe won on away goals.'' Red Star Belgrade won 2–0 on aggregate. Rennes won 4–3 on aggregate. ''Palermo 1–1 Mladá Boleslav on aggregate. Mladá Boleslav won 4–2 on penalties.'' Anderlecht won 2–1 on aggregate. ''Fiorentina 2–2 Groningen on aggregate. Fiorentina won 4–3 on penalties.'' Bolton Wanderers won 2–1 on aggregate. ''Aris 2–2 Real Zaragoza on aggregate. Aris won on away goals.'' AEL won 3–2 on aggregate. Bordeaux won 4–3 on aggregate. AZ won 1–0 on aggregate. Braga won 5–2 on aggregate. Bayer Leverkusen won 5–4 on aggregate.