Serena Williams defeated Jennifer Capriati in the final, 6–1, 6–7(7–9), 6–3 to win the women's singles tennis title at the 2001 Canadian Open. Martina Hingis was the two-time reigning champion, but chose not to participate that year.

Seeds

The top eight seeds received a bye into the second round.

Draw

Finals

Top half

Section 1

Section 2

Bottom half

Section 3

Section 4

Qualifying

Qualifying seeds

• # 🇺🇸 Jennifer Hopkins (qualified) • # 🇯🇵 Shinobu Asagoe (qualifying competition, lucky loser) • # 🇦🇺 Evie Dominikovic (qualifying competition, lucky loser) • # 🇦🇺 Alicia Molik (first round) • # 🇹🇳 Selima Sfar (qualified) • # 🇫🇷 Émilie Loit (first round) • # 🇺🇸 Sandra Cacic (qualifying competition, retired) • # 🇺🇸 Jill Craybas (first round) • # 🇩🇪 Anca Barna (first round) • # 🇪🇸 Nuria Llagostera Vives (qualifying competition) • # 🇺🇸 Marissa Irvin (qualified) • # 🇦🇷 María Emilia Salerni (first round) • # Silvija Talaja (qualifying competition) • # 🇮🇹 Adriana Serra Zanetti (qualified) • # Katarina Srebotnik (first round) • # 🇷🇺 Anastasia Myskina (qualifying competition)

Qualifiers

• # 🇺🇸 Jennifer Hopkins • # 🇰🇿 Irina Selyutina • # 🇺🇸 Marissa Irvin • # 🇺🇸 Samantha Reeves • # 🇹🇳 Selima Sfar • # 🇺🇸 Mashona Washington • # Wynne Prakusya • # 🇮🇹 Adriana Serra Zanetti

Lucky losers

• # 🇯🇵 Shinobu Asagoe • # 🇦🇺 Evie Dominikovic

Qualifying draw

First qualifier

Second qualifier

Third qualifier

Fourth qualifier

Fifth qualifier

Sixth qualifier

Seventh qualifier

Eighth qualifier