Venus Williams defeated Lindsay Davenport in the final, 6–4, 7–5 to win the women's singles tennis title at the 2000 US Open. It was her second major singles title, and she became the second woman to win Wimbledon, the Olympics, and the US Open in the same season (after Steffi Graf in 1988). Serena Williams was the defending champion, but was defeated in the quarterfinals by Davenport in a rematch of the previous year's semifinal. This was the first major appearance for future French Open champion Francesca Schiavone. She entered the main draw as a qualifier and lost to Jelena Dokić in the third round.

Seeds

• # Martina Hingis (semifinals) • # 🇺🇸 Lindsay Davenport (final) • # 🇺🇸 Venus Williams (champion) • # 🇫🇷 Mary Pierce (fourth round, retired because of a shoulder injury) • # 🇺🇸 Serena Williams (quarterfinals) • # 🇺🇸 Monica Seles (quarterfinals) • # 🇪🇸 Conchita Martínez (third round) • # 🇫🇷 Nathalie Tauziat (quarterfinals) • # 🇪🇸 Arantxa Sánchez Vicario (fourth round) • # 🇩🇪 Anke Huber (quarterfinals) • # 🇫🇷 Sandrine Testud (fourth round) • # 🇷🇺 Anna Kournikova (third round) • # Amanda Coetzer (third round) • # 🇧🇪 Dominique Van Roost (second round) • # 🇺🇸 Jennifer Capriati (fourth round) • # 🇫🇷 Julie Halard-Decugis (first round)

Qualifying

Draw

Finals

Top half

Section 1

Section 2

Section 3

Section 4

Bottom half

Section 5

Section 6

Section 7

Section 8