Gustavo Kuerten defeated Andre Agassi in the final, 6–4, 6–4, 6–4 to win the singles tennis title at the 2000 Tennis Masters Cup. With the win, Kuerten became the year-end No. 1, and became the first South American to make the achievement. Pete Sampras was the defending champion, but was defeated in the semifinals by Kuerten.

Seeds

• # 🇷🇺 Marat Safin (semifinals) • # 🇧🇷 Gustavo Kuerten (champion) • # 🇺🇸 Pete Sampras (semifinals) • # 🇸🇪 Magnus Norman (round robin) • # 🇷🇺 Yevgeny Kafelnikov (round robin) • # 🇦🇺 Lleyton Hewitt (round robin) • # 🇪🇸 Àlex Corretja (round robin) • # 🇺🇸 Andre Agassi (final)

Alternate

• # 🇸🇪 Thomas Enqvist (Did not play)

Draw

Finals

Red group

''Standings are determined by: 1. number of wins; 2. number of matches; 3. in two-players-ties, head-to-head records; 4. in three-players-ties, percentage of sets won, or of games won; 5. steering-committee decision.''

Green group

''Standings are determined by: 1. number of wins; 2. number of matches; 3. in two-players-ties, head-to-head records; 4. in three-players-ties, percentage of sets won, or of games won; 5. steering-committee decision.''