The 2000 NCAA Division I baseball tournament was played at the end of the 2000 NCAA Division I baseball season to determine the national champion of college baseball. The tournament concluded with eight teams competing in the College World Series, a double-elimination tournament in its fifty fourth year. Sixteen regional competitions were held to determine the participants in the final event, with each winner advancing to a best of three series against another regional champion for the right to play in the College World Series. Each region was composed of four teams, resulting in 64 teams participating in the tournament at the conclusion of their regular season, and in some cases, after a conference tournament. The fifty-fourth tournament's champion was LSU, coached by Skip Bertman. The Most Outstanding Player was Trey Hodges of LSU.

National seeds

Bold indicates CWS participant.

Regionals and super regionals

Bold indicates winner.

• indicates host.

Columbia Super Regional

Baton Rouge Super Regional

Atlanta Super Regional

Clemson Super Regional

Houston Super Regional

Tallahassee Super Regional

Austin Super Regional

Hosted by Texas at Disch–Falk Field in Austin, Texas

Palo Alto Super Regional

Notes on tournament field

College World Series

Participants

Results

Bracket

Game results

All-Tournament Team

The following players were members of the College World Series All-Tournament Team.

Notable players