Mary Pierce defeated Conchita Martínez in the final, 6–2, 7–5 to win the women's singles tennis title at the 2000 French Open. It was her second and last major singles title. Pierce became the first Frenchwoman to win the title since Françoise Dürr in 1967 and is the most recent Frenchwoman to win the tournament. Steffi Graf was the reigning champion, but she retired from professional tennis in August 1999. This was also the French Open main-draw debut of future champion Anastasia Myskina; she lost to Cara Black in the first round.

Seeds

• # Martina Hingis (semifinals) • # 🇺🇸 Lindsay Davenport (first round) • # 🇺🇸 Monica Seles (quarterfinals) • # 🇺🇸 Venus Williams (quarterfinals) • # 🇪🇸 Conchita Martínez (final) • # 🇫🇷 Mary Pierce (champion) • # 🇫🇷 Nathalie Tauziat (third round) • # 🇪🇸 Arantxa Sánchez Vicario (semifinals) • # Amanda Coetzer (third round) • # 🇫🇷 Sandrine Testud (third round) • # 🇩🇪 Anke Huber (fourth round) • # 🇫🇷 Julie Halard-Decugis (first round) • # 🇫🇷 Amélie Mauresmo (fourth round) • # 🇷🇺 Anna Kournikova (second round) • # 🇺🇸 Jennifer Capriati (first round) • # 🇦🇹 Barbara Schett (fourth round)

Qualifying

Draw

Key

Finals

Earlier rounds

Section 1

Section 2

Section 3

Section 4

Section 5

Section 6

Section 7

Section 8