Thomas Enqvist defeated Tim Henman in the final, 7–6(7–5), 6–4 to win the singles tennis title at the 2000 Cincinnati Masters. Pete Sampras was the defending champion, but lost in the third round to Henman.

Seeds

Draw

Finals

Top half

Section 1

Section 2

Bottom half

Section 3

Section 4

Qualifying

Qualifying seeds

• # 🇮🇹 Gianluca Pozzi (qualified) • # 🇧🇾 Vladimir Voltchkov (qualified) • # 🇨🇿 Martin Damm (qualified) • # George Bastl (first round) • # 🇮🇹 Davide Sanguinetti (first round) • # Peter Wessels (first round) • # 🇮🇹 Andrea Gaudenzi (qualifying competition, lucky loser) • # 🇫🇷 Cyril Saulnier (first round) • # Cecil Mamiit (qualified) • # 🇺🇸 Bob Bryan (qualified) • # 🇸🇰 Ján Krošlák (first round) • # Fernando González (qualifying competition) • # Kevin Ullyett (first round, retired due to a knee injury) • # 🇬🇧 Arvind Parmar (qualifying competition) • # 🇫🇷 Jean-René Lisnard (first round) • # Marcos Ondruska (qualifying competition)

Qualifiers

• # 🇮🇹 Gianluca Pozzi • # 🇧🇾 Vladimir Voltchkov • # 🇨🇿 Martin Damm • # Neville Godwin • # 🇺🇸 Taylor Dent • # 🇫🇷 Lionel Roux • # Cecil Mamiit • # 🇺🇸 Bob Bryan

Lucky loser

Special exempt

Qualifying draw

First qualifier

Second qualifier

Third qualifier

Fourth qualifier

Fifth qualifier

Sixth qualifier

Seventh qualifier

Eighth qualifier