The 2000–01 OHL season was the 21st season of the Ontario Hockey League. The Guelph Storm moved from the Guelph Memorial Gardens to the Guelph Sports and Entertainment Centre at the start of the season. The Owen Sound Platers were renamed to the Owen Sound Attack Twenty teams each played 68 games. The Ottawa 67's won the J. Ross Robertson Cup, defeating the Plymouth Whalers.

Regular season

Final standings

Note: DIV = Division; GP = Games played; W = Wins; L = Losses; T = Ties; OTL = Overtime losses; GF = Goals for; GA = Goals against; PTS = Points; x = clinched playoff berth; y = clinched division title; z = clinched conference title

Eastern conference

Western conference

Scoring leaders

Playoffs

Conference quarterfinals

Eastern conference

Western conference

Conference semifinals

Conference finals

J. Ross Robertson Cup finals

J. Ross Robertson Cup Champions Roster

All-Star teams

First team

Second team

Third team

Awards

2001 OHL Priority Selection

On May 5, 2001, the OHL conducted the 2001 Ontario Hockey League Priority Selection. The Mississauga IceDogs held the first overall pick in the draft, and selected Patrick O'Sullivan from the USA U17 team. O'Sullivan was awarded the Jack Ferguson Award, awarded to the top pick in the draft. Below are the players who were selected in the first round of the 2001 Ontario Hockey League Priority Selection.