The 2000–01 Northern Premier League season was the 33rd in the history of the Northern Premier League, a football competition in England. Teams were divided into two divisions; the Premier and the First.

Premier Division

The Premier Division featured three new teams:

League table

Results

Division One

Division One featured four new teams:

League table

Results

Promotion and relegation

In the thirty-third season of the Northern Premier League Stalybridge Celtic (as champions) were automatically promoted to the Football Conference. Leek Town and Spennymoor United were relegated to the First Division; these two clubs were replaced by First Division winners Bradford Park Avenue, second placed Vauxhall Motors and readmitted Burton Albion (returning from the Southern League Premier Division. In the First Division Winsford United and Congleton Town left the League at the end of the season and were replaced by newly admitted Rossendale United and Ossett Albion.

Cup Results

Challenge Cup: Teams from both leagues. President's Cup: 'Plate' competition for losing teams in the NPL Cup. Chairman's Cup: 'Plate' competition for losing teams in the NPL Cup. Peter Swales Shield: Between Champions of NPL Premier Division and Winners of the NPL Cup.