The 2000–01 European Challenge Cup was the fifth year of the European Challenge Cup, the second tier rugby union cup competition below the Heineken Cup. The tournament was held between October 2000 and May 2001.

Pool stage

Pool 1

Pool 2

Pool 3

Pool 4

Pool 5

Pool 6

Pool 7

Pool 8

Knockout stage

Quarter-finals

Semi-finals

Final

Harlequins: J. Williams, Gollings, Greenwood, Greenstock, O'Leary, Burke, M. Powell, Leonard, Wood, Dawson, Morgan, White-Cooper, Sanderson, Wilson, Winters. Replacements: Friday, Chalmers, Jennings, Starr, Fuga, Codling, Jenkins. Narbonne: Corletto, Joubert, Douy, A. Stoica, Rouch, Quesada, Sudre, Martinez, Ledesma, Pucciarello, Gaston, Merle, Furet, Raynaud, Reid. Replacements: Racine, Poux, Plateret, Mathieu, Azema, Rosalen, Buada. Referee: Nigel Whitehouse (Wales)