The 2000–01 Divizia B was the 61st season of the second tier of the Romanian football league system. The format has been maintained to two series, each of them having 18 teams. At the end of the season, the winners of the series promoted to Divizia A and the last six places from both series relegated to Divizia C. Two more teams relegated this season due to the reduction of team in the next season, from 36 (2x18) to 32 (2x16). A promotion play-off was played between the 13th and 14th-placed in the Divizia A and the runners-up of the Divizia B series.

Team changes

To Divizia B

Promoted from Divizia C Relegated from Divizia A

From Divizia B

Relegated to Divizia C Promoted to Divizia A

Note (**)

Hondor Agigea promoted but due to financial problems sold its place to Metalul Plopeni.

Renamed teams

Poiana Câmpina was renamed as Dinamo Poiana Câmpina and started to be the second squad of Dinamo București. Electro-Bere Craiova was renamed as Electro Craiova. Flacăra Râmnicu Vâlcea was renamed as FCM Râmnicu Valcea.

League map

League tables

Seria I

Seria II

Divizia A play-off

The 13th and 14th-placed teams of the Divizia A faced the 2nd-placed teams of the Divizia B. Note: FC Baia Mare sold their 2001–02 Divizia A place to FCM Bacău.

Top scorers