The 2000–01 Coppa Italia was the 54th edition of the tournament. The tournament was won by Fiorentina, for their 6th title, also qualifying for the UEFA Cup in the progress. It was Fiorentina's second title in recent years, having also won the 1995–96 edition of the tournament. Fiorentina defeated Parma in the two-legged final on an aggregate score of 2–1.

Seedings and format

In the 2000–01 season, Coppa Italia featured 48 teams competing: all 18 clubs from Serie A, all 20 clubs from Serie B, the 4 clubs relegated from Serie B in the 1999–00 season, the 4 playoff runners-up from the 1999–00 Serie C1 season, and the 2 finalists of the 1999–00 Serie C Coppa Italia competition. The format for pairings were:

Group 1

Group 2

Group 3

Group 4

Group 5

Group 6

Group 7

Group 8

Knockout stage

Final

First leg

Second leg

Fiorentina won 2–1 on aggregate.

Top goalscorers