The 1965 Australian Championships was a tennis tournament that took place on outdoor Grass courts at the Kooyong Lawn Tennis Club, Melbourne, Australia from 22 January to 1 February. It was the 53rd edition of the Australian Championships (now known as the Australian Open), the 15th held in Melbourne, and the first Grand Slam tournament of the year. The singles titles were won by Australians Roy Emerson and Margaret Smith.

Champions

Men's singles

🇦🇺 Roy Emerson defeated 🇦🇺 Fred Stolle 7–9, 2–6, 6–4, 7–5, 6–1

Women's singles

🇦🇺 Margaret Smith defeated 🇧🇷 Maria Bueno 5–7, 6–4, 5–2 retired

Men's doubles

🇦🇺 John Newcombe / 🇦🇺 Tony Roche defeated 🇦🇺 Roy Emerson / 🇦🇺 Fred Stolle 3–6, 4–6, 13–11, 6–3, 6–4

Women's doubles

🇦🇺 Margaret Smith / 🇦🇺 Lesley Turner defeated 🇦🇺 Robyn Ebbern / 🇺🇸 Billie Jean Moffitt, 1–6, 6–2, 6–3

Mixed doubles

🇦🇺 Robyn Ebbern / 🇦🇺 Owen Davidson and 🇦🇺 Margaret Smith / 🇦🇺 John Newcombe (final not played)